Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Indocyanine-enhanced mouse model of bleomycin-induced lung fibrosis with hallmarks of progressive emphysema

doi: 10.1152/ajplung.00180.2022

Figure Lengend Snippet: Inflammatory cells profiling, neutrophil elastase, and TGF-β1 levels in the BAL fluid. Leukocyte infiltration of the BALF from differently treated (Sal+ICG, BLM+ICG, Sal, and BLM) groups. Time-dependent variation in the abundance of total white blood cells (WBC; A ), neutrophils ( B ), macrophages ( C ), lymphocytes ( D ), and eosinophils ( E ). Data are the means ± SE of three independent experiments. Two-way ANOVA with Sidak’s correction for multiple comparisons was applied to the following number of animals ( n ): Sal, Sal+ICG, and BLM groups, n = 5 for each time point; BLM+ICG groups, n = 12 for each time point; * P < 0.05, ** P < 0.01, BLM+ICG vs. Sal+ICG; # P < 0.05 BLM+ICG vs. BLM-only group. F : neutrophil elastase levels measured in the BAL of Sal+ICG, BLM+ICG, Sal, and BLM mice at days 7, 14 , and 21 . G : TGF-β1 measured in the BAL of Sal+ICG, BLM+ICG, Sal, and BLM mice at days 7, 14 , and 21 . Data, expressed as pg/ml, are the means ± SE of three independent replicates. Two-way ANOVA with Tukey’s test for multiple comparisons was used to compare treatment/control pairs (BLM+ICG vs. Sal+ICG group and BLM vs. Sal) at each of the indicated time points. Statistical analysis was applied to the following number of animals ( n ): Sal, Sal+ICG, and BLM groups, n = 5 for each time point; BLM+ICG groups, n = 12 for each time point. **in black P < 0.01 BLM+ICG vs. Sal+ICG; *in red P < 0.05 BLM vs. Sal; ## P < 0.01 BLM+ICG vs. BLM group. BAL, bronchoalveolar lavage; BLM, bleomycin; ICG, indocyanine green; Sal, saline.

Article Snippet: Murine macrophage (RAW 264.7, ATCC Cat. No. TIB-71, RRID:CVCL_0493) and epithelial (LA-4, ATCC Cat. No. CCL-196, RRID:CVCL_3535) cell lines were purchased from ATCC and grown in T-75 flasks (Corning) until they reached the 80% of confluence.

Techniques: Control, Saline

Journal: Cell host & microbe

Article Title: Systematic reconstruction of an effector-gene network reveals determinants of Salmonella cellular and tissue tropism.

doi: 10.1016/j.chom.2021.08.012

Figure Lengend Snippet: Figure 3. Identification of a minimal effector- gene network that supports intracellular Sal- monella replication and macrophage survival in vitro (A) Fold replication of the indicated STm strains in infected H1299, Hela, HCT116, and T84 cells. Sta- tistical significance was determined as in Figure 1B. (B–D) Confocal microscopy and TEM images of H1299 cells infected with the indicated GFP-ex- pressing STm strains for 18 h. The nucleus (blue) and endogenous Lamp1 (red) are shown. Arrowheads show the SCV membrane with multiple bacteria enclosed. See also Figure S3.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LB broth Fisher Scientific Cat#BP9722 LB agar Fisher Scientific Cat#BP9724 Nutrient Broth Becton Dickinson Cat#234000 Paraformaldehyde (4%) Alfa Aesar Cat#47392 PBS Sigma Cat#D8537 RPMI Medium 1640 Thermo Fisher Cat#11875 Saponin MP Biomedicals Cat#102855 Sodium Chloride Fisher Scientific Cat#BP358 Streptomycin sulfate Fisher Scientific Cat#BP910 Sucrose Fisher Science Education Cat#S25590 Triton X-100 Fisher Scientific Cat#BP151 Tryptone Becton Dickinson Cat#211705 Yeast Extract Becton Dickinson Cat#212750 Experimental models: Cell lines Hela ATCC CCL-2 RAW264.7 ATCC TIB-71 HCT116 Mendel Lab N/A T84 Winter Lab N/A H1299 Minna Lab N/A Experimental models: Organisms/strains C57BL/6J mice Jackson Lab 000664 129X1/SvJ mice Jackson Lab 000691 C57BL/6J Nramp1G169 mice (Arpaia et al., 2011) N/A Oligonucleotides See Tables S1–S3 for full list of primers used in this study This Study See Tables S1–S3 Recombinant DNA GFP expression plasmid (Murphy et al., 2002) pBBR1MCS-6y mCherry expression plasmid (Burnaevskiy et al., 2013) pDP151 ori(R6K) mobRP4 Cmr Tetr sacRB (Kingsley et al., 1999) pRDH10 Template plasmid for FRT-flanked KanR cassette (Datsenko and Wanner, 2000) pKD4 Red recombinase expression plasmid (Datsenko and Wanner, 2000) pKD46 Helper plasmid to eliminate the KanR cassette (Datsenko and Wanner, 2000) pCP20 downstream region of STm spvR in pRDH10 This Study pNA101 downstream region of STm pipAB in pRDH10 This Study pNA102 downstream region of STm pipB2 in pRDH10 This Study pNA103 downstream region of STm gtgA in pRDH10 This Study pNA104 downstream region of STm sifB in pRDH10 This Study pNA105 downstream region of STm gtgEsseI in pRDH10 This Study pNA106 downstream region of STm steBsseJ in pRDH10 This Study pNA107 downstream region of STm pipD in pRDH10 This Study pNA108 downstream region of STm sseL in pRDH10 This Study pNA109 downstream region of STm gogBsteE in pRDH10 This Study pNA110 downstream region of STm sopD2 in pRDH10 This Study pNA111 downstream region of STm slrp in pRDH10 This Study pNA112 downstream region of STm steA in pRDH10 This Study pNA113 downstream region of STm steD in pRDH10 This Study pNA114 downstream region of STm sspH2 in pRDH10 This Study pNA115 downstream region of STm sseK2 in pRDH10 This Study pNA116 (Continued on next page) e4 Cell Host & Microbe 29, 1531–1544.e1–e9, October 13, 2021

Techniques: In Vitro, Infection, Confocal Microscopy, Membrane, Bacteria